Spirochrome SiR-微管蛋白试剂盒：一键解锁活细胞动态成像

SiR-微管蛋白是基于微管结合药物多西他赛的。SiR-微管蛋白具有荧光特性、细胞通透性，并且对微管具有很高的特异性。SiR-微管蛋白能够在不需要基因操作或过表达的情况下对内源性微管进行染色。其远红光发射最大限度地减少了光毒性以及样本的自发荧光。SiR-微管蛋白与绿色荧光蛋白（GFP）和/或 m-樱桃荧光蛋白兼容。可以使用标准的 Cy5 滤光片组进行成像。SiR-微管蛋白可用于活细胞和组织的宽场、共聚焦、SIM 或 STED 成像。探针数量允许进行 50 - 200 次染色实验。

作为Spirochrome在中国的区域总代理，环球ug登录科技强烈推荐Spirochrome热销产品SiR-微管蛋白试剂盒。产品仅用于科研，不可用于临床诊断。

SiR-微管蛋白试剂盒

光学性质

λabs 652 nm

εmax 1.0×10⁵ mol^-1·cm^-1

λfl 674 nm

Spirochrome的新产品 SPY650-微管蛋白是 SiR-微管蛋白的改进版本。SPY650-微管蛋白在染色细胞时能够发出更亮的信号，并且背景更少，同时仍然兼容超分辨率成像。了解更多关于 SPY650-微管蛋白的信息，请点击这里：SPY650-微管蛋白

*基于以下条件：每次染色实验使用 0.5 - 1 ml 染色液，探针浓度为 0.5 - 1 uM。通过减少体积或探针浓度，可以进一步增加染色实验的次数。

最新引用文章

1、Multiscale imaging of cytokinesis

Hümpfer N., Marin Z., ..., Ewers H.

bioRxiv  |  2025 Sep 25  |  Read Article  

"PrePrint: .. cells were seeded onto Geltrex-coated glass-bottom imaging dishes (Zellkontakt, 6160-30).. They were synchronized by a single 2 mM thymidine block for 22 h and released into full medium containing 100 nM SiR-tubulin and 10 μM verapamil (both Spirochrome, SC002) and incubated for 5 h. Prior to live-cell imaging, medium was exchanged to FluoroBrite DMEM (Gibco, A1896701), supplemented with 100 nM SiR-tubulin and 10 μM verapamil.. Ultrastructure Expansion microscopy (U-ExM) of cytokinetic cells.Ultrastructure Expansion microscopy (U-ExM) .." More...

2、Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics.

Samuele Ghignoli, Valentina De Lorenzi, ..., Francesco Cardarelli

Traffic (Copenhagen, Denmark)  |  2025 Sep 24  |  40988617  |  Read Article  

".. upkeeping medium and after 15 min of incubation, unless otherwise specified, imaging was performed.. For assessing cytoskeletal components depolymerization, cells were treated for 30 min with 10 μM SiR- Tubulin (Spirochrome, cat# SC002 [62]) to label microtubules or with 10 μM SiR- Actin (Spirochrome, cat# SC001 [62]) to label actin filaments.. For these treatments, 10 μM Nocodazole (Sigma- Aldrich, cat# 487928) and 10 μM Latrunculin- .." More...

3、Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformation

Hotta Takashi, Pimm Morgan L., ..., Verhey Kristen J.

bioRxiv  |  2025 Sep 23  |  Read Article    |  Bioz AI Brief 

"PrePrint: that lattice state impacts the accessibility of the Y-αCTT. Previous work showed that localization of A1aY1 shifts from cytosolic or plus ends to the microtubule lattice in cultured cells treated with SiR-Tubulin ( Kesarwani et al. 2020 ), a Taxol derivative that can convert GDP-tubulin into a GTP-like expanded state ( Siahaan et al. 2022 ). We thus hypothesized that lattice expansion drives access" More...

Spirochrome 成立于 2015年，总部位于瑞士，是一家专注于活细胞荧光成像技术的生物科技公司。公司核心技术源于国际权威学术期刊《Nature Chemistry》与《Nature Methods》发表的两项创新研究成果—— SIR与SPY荧光母核。该类染料具有卓越的稳定性、超高荧光信号与极低背景噪声，能够突破传统成像限制，适用于多种超分辨荧光成像技术， 助力科学家开启活细胞微观世界的分子尺度探索时代。

作为Spirochrome在中国的区域总代理，环球ug登录科技有限公司将为中国客户提供全面的Spirochrome产品以及客户订制化服务，欢迎大家随时联系我们。